Fig. 4. Pra-B inhibits TPA-induced AKT phosphorylation in HeLa cells. The cells were pretreated with TPA (50 ng/ml) for 2 h, incubated with Pra-B (10 and 20 μM) for 24 h, and then subjected to Western blotting to analyze the levels of p-AKT/t-AKT, p-ERK/t-ERK, p-JNK/t-JNK and p-p38/t-p38. β-actin was used as an internal control. These results are representative of three independent experiments. The values shown are the mean ± SE. **P<0.01 compared with untreated cells.